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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11018 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: LC/MS-based lipidomics and transcriptomics reveal lipid diversity and regulatory networks underlying intramuscular fat differences in Xingguo grey geese.
    Article Snippet: Intramuscular fat (IMF) serves as a crucial economic indicator of meat quality.. To investigate the heterogeneity of IMF composition and its regulatory mechanisms in Xingguo (XG) geese with varying IMF levels, lipidomics and transcriptomics were utilized.. The analysis of lipid profiles revealed that the predominant lipids in the IMF of XG geese were glycerophospholipids (GPs), followed by glycerides (GLs).

    cDNA Synthesis:

    Article Title: LC/MS-based lipidomics and transcriptomics reveal lipid diversity and regulatory networks underlying intramuscular fat differences in Xingguo grey geese.
    Article Snippet: Intramuscular fat (IMF) serves as a crucial economic indicator of meat quality.. To investigate the heterogeneity of IMF composition and its regulatory mechanisms in Xingguo (XG) geese with varying IMF levels, lipidomics and transcriptomics were utilized.. The analysis of lipid profiles revealed that the predominant lipids in the IMF of XG geese were glycerophospholipids (GPs), followed by glycerides (GLs).

    Article Title: CRISPR‐Induced Mutations of <i>mk2b</i> and <i>mk3</i> Host Proteins Enhance Chikungunya Virus Susceptibility and Modulate Host Immune Responses in Zebrafish
    Article Snippet: .. RNA was isolated using the MagSure All- RNA isolation kit (RnaBio) followed by the synthesis of cDNA using the 1st strand cDNA synthesis kit (TAKARA) as per the manufacturer's instructions. ..

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis.
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    Article Title: Lnc-HZ05 Suppresses Trophoblast Cell Migrasome Formation by Disrupting TGFβ2 Pathway in Unexplained Recurrent Miscarriage.
    Article Snippet: Reverse Transcription and Quantitative Real-Time PCR (RT-qPCR): Total RNAs were extracted from cells or villous tissues with Trizol Reagent (Invitrogen). .. The first strand cDNA synthesis kit (Takara, Kyoto, Japan) was used for synthesis of cDNA. .. The isolated RNAs (800 ng) were converted into cDNAs using the first-strand cDNA synthesis kit (Invitrogen) using the first strand cDNA synthesis kit (Invitrogen).

    Article Title: FHL2 deteriorates IL-1β induced inflammation, apoptosis, and extracellular matrix degradation in chondrocyte-like ATDC5 cells by mTOR and NF-ĸB pathways
    Article Snippet: .. According to the manufacturer’s protocol, 1,000 ng of RNA was reversely transcribed into cDNA using the first-strand cDNA synthesis kit with gDNA Eraser (RR047A, Takara, Japan). ..

    Article Title: Fucosterol exerts an anti-atherosclerotic action via NF-κB and p38/Erk MAPK signaling pathways
    Article Snippet: Total RNAs were isolated from the aortic tissues or HUVECs using TRIzol (Solarbio, Beijing, China). .. Subsequently, cDNA was synthesized using the first strand cDNA synthesis kit (Takara, Dalian, China), and used for PCR amplification in the presence of SYBR Premix Ex Taq (Takara, Dalian, China). ..

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    Article Title: Design, synthesis of next generation indomethacin-based cyclooxygenase-2 inhibitor: An in silico and in vitro investigation.
    Article Snippet: The current study presents a series of indomethacin-phenolic phytochemical hybrids (INDO_PP1 to INDO_PP15) using the Steglich esterification reaction to develop potential antioxidant and anti-inflammatory lead drug candidates.. After the design of the hybrid candidates (INDO_PP1 to INDO_PP15), various bioinformatics and chemoinformatics tools were employed to predict the potency, toxicity, and drug-ability profiles.. The AutoDock 4.2 tool was used for binding efficacy; the GROMACS-2020 and MM/PBSA for stability of docking complexes; the SwissADME for physicochemical and pharmacokinetics; and the Molsoft for drug-likeness prediction.

    Isolation:

    Article Title: CRISPR‐Induced Mutations of <i>mk2b</i> and <i>mk3</i> Host Proteins Enhance Chikungunya Virus Susceptibility and Modulate Host Immune Responses in Zebrafish
    Article Snippet: .. RNA was isolated using the MagSure All- RNA isolation kit (RnaBio) followed by the synthesis of cDNA using the 1st strand cDNA synthesis kit (TAKARA) as per the manufacturer's instructions. ..

    Synthesized:

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis.
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    Article Title: Fucosterol exerts an anti-atherosclerotic action via NF-κB and p38/Erk MAPK signaling pathways
    Article Snippet: Total RNAs were isolated from the aortic tissues or HUVECs using TRIzol (Solarbio, Beijing, China). .. Subsequently, cDNA was synthesized using the first strand cDNA synthesis kit (Takara, Dalian, China), and used for PCR amplification in the presence of SYBR Premix Ex Taq (Takara, Dalian, China). ..

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    SYBR Green Assay:

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis.
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    Article Title: Spermidine Alleviates Sepsis-Induced Acute Lung Injury through AMPK-Mediated Improvement of Necroptosis
    Article Snippet: .. Total cells or tissues RNA was extracted with TRIZOL Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions, the cDNA was synthesized by using the first strand cDNA synthesis kit (Takara Shuzo, Kyoto, Japan). q-PCR was applied using the SYBR Green dye (Takara, Japan) on LightCycler 480 system (Roche, Penzberg, Germany). ..

    Polymerase Chain Reaction:

    Article Title: Fucosterol exerts an anti-atherosclerotic action via NF-κB and p38/Erk MAPK signaling pathways
    Article Snippet: Total RNAs were isolated from the aortic tissues or HUVECs using TRIzol (Solarbio, Beijing, China). .. Subsequently, cDNA was synthesized using the first strand cDNA synthesis kit (Takara, Dalian, China), and used for PCR amplification in the presence of SYBR Premix Ex Taq (Takara, Dalian, China). ..

    Amplification:

    Article Title: Fucosterol exerts an anti-atherosclerotic action via NF-κB and p38/Erk MAPK signaling pathways
    Article Snippet: Total RNAs were isolated from the aortic tissues or HUVECs using TRIzol (Solarbio, Beijing, China). .. Subsequently, cDNA was synthesized using the first strand cDNA synthesis kit (Takara, Dalian, China), and used for PCR amplification in the presence of SYBR Premix Ex Taq (Takara, Dalian, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Design, synthesis of next generation indomethacin-based cyclooxygenase-2 inhibitor: An in silico and in vitro investigation.
    Article Snippet: The current study presents a series of indomethacin-phenolic phytochemical hybrids (INDO_PP1 to INDO_PP15) using the Steglich esterification reaction to develop potential antioxidant and anti-inflammatory lead drug candidates.. After the design of the hybrid candidates (INDO_PP1 to INDO_PP15), various bioinformatics and chemoinformatics tools were employed to predict the potency, toxicity, and drug-ability profiles.. The AutoDock 4.2 tool was used for binding efficacy; the GROMACS-2020 and MM/PBSA for stability of docking complexes; the SwissADME for physicochemical and pharmacokinetics; and the Molsoft for drug-likeness prediction.

    Expressing:

    Article Title: Design, synthesis of next generation indomethacin-based cyclooxygenase-2 inhibitor: An in silico and in vitro investigation.
    Article Snippet: The current study presents a series of indomethacin-phenolic phytochemical hybrids (INDO_PP1 to INDO_PP15) using the Steglich esterification reaction to develop potential antioxidant and anti-inflammatory lead drug candidates.. After the design of the hybrid candidates (INDO_PP1 to INDO_PP15), various bioinformatics and chemoinformatics tools were employed to predict the potency, toxicity, and drug-ability profiles.. The AutoDock 4.2 tool was used for binding efficacy; the GROMACS-2020 and MM/PBSA for stability of docking complexes; the SwissADME for physicochemical and pharmacokinetics; and the Molsoft for drug-likeness prediction.



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